Nedd8 Conjugation Kit

Product information

$438.90

Description

Description

Nedd8 Conjugation Kit is designed for assaying the conjugation of Nedd8 to protein substrates in vitro. In a protein neddylation reaction, Nedd8 is first activated by the Nedd8 activating enzyme APPBP1/UBA3 (E1) in an ATP hydrolysis-dependent manner; activated Nedd8 is then transferred to the Nedd8 conjugating enzyme Ube2M (UbcH12) (E2) by formation of a thiolester bond with the catalytic cysteine residue of UbcH12I; Nedd8-charged Ube2M (UbcH12) can mediate protein neddylation in the presence of an appropriate Nedd8 ligase (E3).

Overview

Overview

20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol, Souce: E. coli recombinant, Buffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol

Additional details

Downloads

Downloads

http://www.ubpbio.com/temp/J3150_Nedd8%20Conjugation%20Kit_Datasheet.pdf

SKU

UBP-J3150

Manufacturer Part Number

J3150

Specification

Human

UOM

1 kit