6xHis-NEMO (UBAN)

Product information

$150.15

Description

Description

6xHis was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO encompassing amino acids 183-339. This fusion protein can be used for in vitro pulldown assays and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. 6xHis-NEMO (UBAN) can be precipitated using Nickel resin. After washing, 6xHis-NEMO (UBAN) and its bound proteins can be eluted by a buffer containing 200 mM imidazole.

Overview

Overview

20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol, Souce: E. coli recombinant, Buffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol

Additional details

Downloads

Downloads

http://www.ubpbio.com/temp/I1520%20I1521_6xHis-NEMO%20(UBAN)_Datasheet.pdf

SKU

UBP-I1520

Manufacturer Part Number

I1520

Specification

Human

UOM

100 µg