Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC)

Product information

$138.60

Description

Description

Boc-LRR-AMC is a fluorogenic substrate for the trypsin – like activity of the 20S proteasome. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.

Overview

Overview

powder, no buffer, Souce: chemical synthesis, Buffer: powder, no buffer

Additional details

Downloads

Downloads

http://www.ubpbio.com/temp/G3100,G3101_Boc-Leu-Arg-Arg-AMC%20(Boc-LRR-AMC)_Datasheet.pdf

SKU

UBP-G3100

Manufacturer Part Number

G3100

UOM

5 mg