Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)
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$150.15
Description
Description
Ac-ANW-AMC is a fluorogenic substrate of the chymotrypsin-like activity of proteasomes. This substrate is preferentially cleaved by immunoproteasomes compared to constitutive proteasomes. The AMC fluorescence can be detected by a fluorimeter or a plate reader using excitation/emission wavelengths at 360 nm/460 nm, respectively.
This substrate can be used to determine the chymotrypsin-like activity of immunoproteasomes. Working concentration is 50 - 200 µM.
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