Ubiquitin (L73P)

Product information

$150.15

Description

Description

Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. Studies have shown that polyUb chains formed by the Ub(L73P) mutant are resistant to deubiquitination catalyzed by many deubiquitination enzymes.

Overview

Overview

20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol, Souce: E. coli recombinant, Buffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol

Additional details

Downloads

Downloads

http://www.ubpbio.com/temp/E1930_Ubiquitin%20(L73P)_Datasheet.pdf

SKU

UBP-E1930

Manufacturer Part Number

E1930

Specification

Human

UOM

1 mg