GST-Ubiquitin (I44A)

Product information

$150.15

Description

Description

Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. I44 is one of the key residues of the hydrophobic patch on Ub surface. The I44A mutant inhibits the interactions of Ub with many Ub-binding domains.

Overview

Overview

20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol, Souce: E. coli recombinant, Buffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol

Additional details

Downloads

Downloads

http://www.ubpbio.com/temp/E1920_GST-Ubiquitin%20(I44A)_Datasheet.pdf

SKU

UBP-E1920

Manufacturer Part Number

E1920

Specification

Human

UOM

1 mg