N-terminal Biotin-Ubiquitin

Product information

$3,087.00

Description

Description

A single biotin moiety is conjugated on the N-terminus of ubiquitin. All lysine residues of ubiquitin are still available for formation of polyubiquitin chains. The extremely high binding affinity between biotin and streptavidin allows the use of N-terminal biotin-ubiquitin for the following in vitro assays: 1) rapid and efficient purification of polyubiquitinated substrate proteins; 2) monitoring substrate ubiquitination using an HRP-conjugated streptavidin antibody; and 3) setting up high throughput FRET assays to monitor substrate ubiquitination in assays screening E3 ubiquitin ligase inhibitors.

Overview

Overview

20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol, Souce: chemical synthesis, Buffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol

Additional details

Downloads

Downloads

http://www.ubpbio.com/temp/E1140%20E1141_Biotin-Ub_Datasheet.pdf

SKU

UBP-E1141

Manufacturer Part Number

E1141

Specification

Human

UOM

1 mg