Fluorescein labeled K63-Ub2

Product information

$334.95

Description

Description

This product contains an authentic K63-linked isopeptide bond, synthesized by using an enzymatic reaction containing UbE1, UbE2N, Ub(K63R) and Ub-fluorescein. Fluorescein is labeled on the C-terminus of Ub by mutating glycine 76 to a cysteine. In fluorescein labeled K63 Ub2, fluorescein is labeled on the proximal Ub. It is an excellent tool for monitoring deubiquitination that can be visualized by in-gel fluorescent detection of the production of fluorescein labeled monoUb (excitation at ~490 nm, and see product data sheet for examples). The assay is more quantitative and sensitive than immunoblotting of Ub. The detection limit is 10 ng or less.

Overview

Overview

20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol, Souce: E. coli recombinant, Buffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol

Additional details

Downloads

Downloads

https://www.ubpbio.com/temp/D2110-Fluorescein%20Labeled%20K63%20Ub2_Datasheet.pdf

SKU

UBP-D2110

Manufacturer Part Number

D2110

Specification

Human

UOM

25 µg