Diaplate 96 Sample 3.2uL Dialysis Plate (3 Plates/PK)

The disposable Diaplate is a 96 well micro-dialysis plate for very small volumes of up to a maximum of 3.2µL. The sample solution is pipetted onto each of the 96 membranes with up to 0.35mL unique dialysis solutions in the wells used for exchange. Dialysis is recommended for 2-3 hours before the dialysis buffer is changed and left for another 2-3 hours to achieve over 90% exchange. Further dialysis steps can be carried out as required. Incubation on a rocking table for speed and a moisture chamber for full humidification can be applied to the experiment if desired. The Diaplate™ kit includes: Diaplate with 200 Micron Pressure Adhesive Spacer, 200 Micron UVP Cover Film, engraved UV Protective Cover and a sealing Paddle.

Owing to the high position of the membrane and the UV transparent cover, samples on Diaplate can be observed by microscopy, fluorimetry, turbidimetry and light scattering. Diaplate is therefore ideal for crystallization by dialysis by changing ionic strength, or simply offering different kinetics to vapour diffusion. Diaplate is also suitable for ligand exchange, slow controlled addition of cryoprotectant and for protein stability screening.

Features of Diaplate:

• Simple handling, ready to use, easy sample recovery
• Complete kit, no additional materials required
• Pipetting can be performed manually or using a robot
• Ideal for the dialysis of up to 3.2µL protein solution
• No cross contamination or leakage between wells guaranteed
• 96 individual regenerated cellulose membranes; sulphur and heavy metal free
• Molecular weight cut off of 10,000 Daltons
• Protein crystallization applications
• Low protein binding and high protein retention
• Dialysis complete in 2-4 hours
• Gentle and efficient dialysis without disturbing valuable protein complexes
• High throughput; simultaneous processing of 96 experiments
• Reliable and uniform, stable construction
• Single use - disposable device; no cleaning required