Calibre Scientific Global

This is the SMALL (0.3 mg) vial, with enough compound to set up 5 x 96-well plates of your protein in complex with 10 mM Crystallophore No1 (the recommended concentration for crystallisation). The small 0.3 mg vial contains too little Crystallophore No1 for soaking into crystals for phasing experiments. Polyvalan Crystallophore N^o1(patent pending) is a luminescent Terbium complex that is an effective nucleant and provides significant isomorphous and anomalous phasing power. It has been used in a wide range of structure solutions since it's launch, including novel protein complexes and integral membrane proteins. A recent review in J. Appl. Cryst. highlights its wide range of applications for increasing your crystal structure success rates. You can also read more about how Crystallophore N^o1 works here

FORMING

Crystallophore N^o1 acts as a nucleating agent and improves crystal quality of pre-existing hits. As shown right for T5 phage with and without Crystallophore N^o1 (top right).

FINDING

Crystallophore N^o1 luminesces strongly in the visible region under UV excitation making detecting crystals in drops and loops easy. See middle right.

PHASING

Terbium is a lanthanide and therefore has significant potential for use as an anomalous scatterer and in traditional isomorphous replacement. When Crystallophore N^o1 has been used as a nucleant, it is often found bound to the protein thus providing high quality phasing power.

NEXT STEPS

To help you build the structure you can download the pdb or cif format co-ordinate files for the Crystallophore N^o1 complex. Kindly provided by Polyvalan.

READ ALL ABOUT IT

The development work leading to Crystallophore No1 has now been published in:

Engilberge, S., Riobé, F., Di Pietro, S., Lassalle, L., Coquelle, N., Arnaud, C.-A., Pitrat, D., Mulatier, J.-C., Madern, D., Breyton, C., Maury, O. and Girard, E. Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effect, phasing properties and luminescence. Chemical Science 8: 5909-5917 (2017).

(bottom right) P. horikoshii Protease 1 electron density from SAD phasing using the Terbium L_III absorption edge. Crystal grown in 10 mM and subsequently soaked in 100 mM crystallophore N^o1.

Each small vial ccontains 0.3 mg of the complex, enough to set up 5 96-well plates of your target protein in complex with 10 mM Crystallophore N^o1 (recommended concentration). Shipped at room temperature. The dry powder will keep for upto 1 year in a sealed tube at room temperature in dry conditions. Solutions of Crystallophore N^o1 can be kept for up to 2 months in a sealed tube. Solutions of the compound mixed with your target protein should be used immediately.

Crystallophore No 1 (small vial, 0.3mg)

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